Antisense GAP-43 Inhibits the Evoked Release of Dopamine from PC12 Cells
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چکیده
منابع مشابه
Chorein Sensitive Dopamine Release from Pheochromocytoma (PC12) Cells.
BACKGROUND Chorein, a protein supporting activation of phosphoinositide 3 kinase (PI3K), participates in the regulation of actin polymerization and cell survival. A loss of function mutation of the chorein encoding gene VPS13A (vacuolar protein sorting-associated protein 13A) leads to chorea-acanthocytosis (ChAc), a neurodegenerative disorder with simultaneous erythrocyte akanthocytosis. In blo...
متن کاملFactors influencing GAP-43 gene expression in PC12 pheochromocytoma cells.
We have studied factors controlling message levels for the neuronal growth- and plasticity-associated protein, GAP-43. Following exposure of PC12 cells to various effectors, cytoplasmic RNA was isolated and analyzed by Northern transfer and autoradiography using a GAP-43 cDNA probe. Induction by NGF is apparent after 3 hr exposure and reaches maximal levels at 24 hr. Beyond 24 hr, levels remain...
متن کاملStable gene silencing of synaptotagmin I in rat PC12 cells inhibits Ca2+-evoked release of catecholamine.
Synaptotagmin (syt) I is a Ca2+-binding protein that is well accepted as a major sensor for Ca2+-regulated release of transmitter. However, controversy remains as to whether syt I is the only protein that can function in this role and whether the remaining syt family members also function as Ca2+ sensors. In this study, we generated a PC12 cell line that continuously expresses a short hairpin R...
متن کاملStable gene silencing of synaptotagmin I in rat PC12 cells inhibits Ca -evoked release of catecholamine
Moore, Johnnie M., Jason B. Papke, Anne L. Cahill, and Amy B. Harkins. Stable gene silencing of synaptotagmin I in rat PC12 cells inhibits Ca -evoked release of catecholamine. Am J Physiol Cell Physiol 291: C270–C281, 2006. First published February 8, 2006; doi:10.1152/ajpcell.00539.2005.—Synaptotagmin (syt) I is a Ca -binding protein that is well accepted as a major sensor for Ca -regulated re...
متن کاملThe prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
BACKGROUND Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of "tonifying qi and strengthening spleen" group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and "eliminating phlegm and strengthening intelligence" group (EPSI) composed of Polygala tenuif...
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ژورنال
عنوان ژورنال: Journal of Neurochemistry
سال: 1993
ISSN: 0022-3042,1471-4159
DOI: 10.1111/j.1471-4159.1993.tb03194.x